Journal of neuroscwnce methodv, 31 1990 8991 89 elsevier nsm 01028 a pipette holder for use in patch clamp measurements henk p. Design of the epc9, a computercontrolled patchclamp. The epc 10 usb patch clamp amplifiers are successors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990123, and the well known epc 10 patch clamp amplifiers, which replaced the epc 9s in 2002. Modalities of distortion of physiological voltage signals by.
A fourchannel integrated patchclamp amplifier with currentclamp capability. The epc 800 usb is the most flexible patch clamp amplifier heka has ever produced in that it is a standalone amplifier that can be combined with any existing adda interface and. Isolated amplifier clinical use voltammetric amplifiers. A growing number of experimental studies have used patchclamp amplifiers pcas in the currentclamp cc mode to investigate classical excitability. This amplifier is truly a unique hybrid patch clamp amplifier with its design and featureset primarily based upon the manually controlled epc 8. Patch clamp recordings in the whole cell configuration, and single channel recordings in the cell attached configuration, can be performed with planar patch clamp chips. A growing number of experimental studies have used patch clamp amplifiers pcas in the current clamp cc mode to investigate classical excitability. Warner instruments is known throughout the world as an innovative engineering company that designs, develops, and manufactures biomedical devices for the electrophysiological, cellular, and neurological sciences. World precision instruments amplifiers patch clamp ipa. Similarly in the epc9, digital artifacts are expected only when parameters are changed. Basic electrical properties of in situ endothelial cells of.
The epc9 patchclamp amplifier is a digitally controlled analog device for recording currents in membrane patches and in small cells. Bmi surplus, an industry leader in quality used test equipment, that is in stock and ready to ship, offers a large inventory of used amplifiers, power amplifiers, differential amplifiers, bipolar and many more used amplifiers such as physik instrumente pi p263 high voltage amplifier, tektronix, inc am502 differential amplifier, stanford research sr552 bipolar preamp, crown. Axon multiclamp 700a patch clamp amplifier, two cv7a. Patch pipettes were pulled from borosilicate glass tubes gc 150. Output signals were filtered at 3 khz and digitized on. Email to friends share on facebook opens in a new window or tab share on twitter opens in a new window or tab. Amplifiers, stimulators and filters electrophysiology. Patchclamp amplifiers on a chip pubmed central pmc. Employing the discontinuous single electrode voltage clamp dsevc technique is a very useful procedure to. Heka is now shipping epc 10 usb patch clamp amplifiers. They are equipped with a bnc connector to fit to the headstages of the patch clamp amplifiers epc 10 usb, epc 10, epc 9, epc 800 usb, epc 8, epc 7 or potentiostat pg 300. An extensive evaluation of the possible alterations affecting physiological voltage signals recorded with patch clamp amplifiers pcas working in the current clamp cc mode was carried out by following a modeling approach. Increased current injection capability by a factor of 5. The epc 8 is the successor of the classical epc 7 and produced by heka, the first manufacturer of patchclamp amplifiers in the world epc 5.
The patchclamp amplifier is a ubiquitous tool for characterizing ionchannel activity. They are equipped with a bnc connector to fit to the headstages of the patch clamp amplifiers epc 10, epc 9, epc 8, epc 7 or potentiostat pg 300. The ipa and double ipa integrated patch clamp amplifiers are computercontrolled single or dualheadstage amplifiers optimized for wholecell recording applications. An extensive evaluation of the possible alterations affecting physiological voltage signals recorded with patchclamp amplifiers pcas working in the currentclamp cc mode was carried out by following a modeling approach. Has anyone set up a patch clamp rig with used equipment from. The porta patch is routinely provided with an axonmdc multiclamp amplifier or an epc 10 amplifier from heka together with a computer and the software packages. Technical support patch clamp amplifiers, potentiostats.
They are used with various headstages, suitable q series electrode holders and glass microelectrodes purchased separately to measure corresponding currents in single channel measurements or whole cell studies. Based on the excellent design of the epc 7 improvements and extensions were made which make the epc 8 a worthy successor of the epc 7. A miniaturized multiclamp cmos amplifier for intracellular. Bnc adapter with pin for htype fitting g23 instruments.
The procedure for wholecell voltage clamping using. Heka elektronik epc 10 usb hardware manual pdf download. Pulse pulsefit software for electrophysiological research. Ypey 1 i department of phvstolog and phystologwal physws, unwersm of lemen. Modalities of distortion of physiological voltage signals by patchclamp amplifiers.
A detailed stepbystep description of the standard patch clamp protocol and labome survey results for vibratomes and patch clamp amplifiers. Axon instruments patchclamp amplifiers molecular devices. The epc 9 contains no processor in itself, but relies. Fully integrated patch clamp amplifier and data acquisition system ensures quick and easy setup optimized for wholecell patch clamp recordings in.
Has anyone set up a patch clamp rig with used equipment from ebay or other used equipment sourcese. Fully integrated patch clamp amplifier and data acquisition system ensures quick and easy setup optimized for wholecell patch clamp recordings in tissue slices, adherent or dissociated cells. Modulation by bicuculline and penicillin of the block by t. Epc 10 usb heka patch clamp amplifiers warner instruments. The model 2410 acts as a virtual cell when attached to your amplifier headstage. It is also very useful to test if your patch clamp is functioning properly without having to worry about connections and pipette problems. Imaging extension for patchmaster, potmaster and chartmaster. Introduction the patch clamp is a laboratory technique in electrophysiology that allows investigation of the electrical excitability of neurons and the functional properties and densities of ion channels. The intan clamp system allows users to perform single amplifier or multiamplifier patch clamp electrophysiology experiments with small, affordable hardware and free, opensource software.
Therefore, you can operate various setups with different amplifiers and purposes in a laboratory with the same software. Axopatch patch clamp amplifier is the premier microelectrode amplifier for ultralow noise patch clamp recordings. These workhorse patch clamp amplifiers by warner instruments are ideal equally for research and teaching applications. The patch clamp amplifier is a ubiquitous tool for characterizing ionchannel activity. Nanion portapatch planar patch clamping automate scientific. With two headstages the multiclamp 700a effectively provides four amplifiers in one unit. The portapatch is routinely provided with an axonmdc multiclamp amplifier or an epc10 amplifier from heka together with a computer and the software packages. Heka acquired instrutech, and offers downloads of igor xops to drive instrutech. Our range of heka and npi patch clamp amplifiers are perfect for carrying out whole cell patch clamp recordings. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is equivalent to a fully equipped recording setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a full featured data acquisition and analysis system. For the screening of pharmaceutical compounds or the characterization of expressed channels, highthroughput patchclamp systems are being developed, where recordings are made from cells in each well of a 96 or 384well plate.
Choosing a compatible microelectrode holder for your headstage. Im preparing to set up a patch rig with used equipment such as epc 7 amplifiers, which are available on ebay. The epc 7 represents roughly the seventh in the series of patch clamp designs in use in the gottingen laboratories of neher and sakmann since their initial experiments and produced by heka as the first manufacturer of patch clamp amplifiers in the world epc 5. Epc 10 patch clamp amplifiers single, double, triple, quadro with builtin lih1600 interfaces optical cable connection the epc 10 usb patch clamp amplifiers single, double, triple, quadro are still maintained epc 10 plus patch clamp amplifiers single, double with builtin itc18 interfaces.
This amplifier is truly a unique hybrid patchclamp amplifier with its design and featureset primarily based upon the manually controlled epc 8. A fiiting to allow all current db and iso range micro electrode holders to be used with heka co. One major problem in whole recording is series resistance. Output signals were filtered at 3 khz and digitized online at 24 khz with a national instruments austin, tx nbmio 16bit ad converter and were stored with pulse fit software heka instruments, bellmore, ny. This allows the user to remotely switch between lownoise patchclamp recording using the axopatchlike headstage circuit and true, fast currentclamp recording using the axoclamplike headstage circuit.
For the screening of pharmaceutical compounds or the characterization of expressed channels, highthroughput patch clamp systems are being developed, where recordings are made from cells in each well of a 96 or 384well plate. This device connects to a host computer through a usb 2. Pdf action potentials recorded with patchclamp amplifiers. It has neither frontpanel controls nor internal trim adjustments. It is a logical successor to the classical epc 7, retaining all of its features but adding a number of capabilities. A patchclamp amplifier, oscilloscope, stimulator, computer, optional external signal filter, and vcr recorder. In proceedings of midwest symposium on circuits and systems 14 ieee, 2011. All amplifiers epc 10, epc 9, epc 8, and epc 7 come with the standard pipette holder for glass capillaries with an outer diameter of 1. Epc 10 usb double triple quadro patch clamp amplifiers.
The portfolio of amplifiers includes axopatch 200b for ultra lownoise singlechannel recordings, multiclamp 700b for wholecell voltageclamp and highspeed currentclamp recordings, and axoclamp 900a for two. The axon instruments series of amplifiers provide bestinclass solutions for the entire range of patchclamp experiments. A dll dynamic link library is available for software developers who are interested in writing their own windows data acquisition software. The epc 10 double and epc 10 triple patch clamp amplifiers are the optimal instruments for performing double or triple patch experiments. Action potentials recorded with patchclamp amplifiers. This model can be used to train students in patch clamp technique, or to improve your patch clamping techniques.
Heka is known for the epc 7 and epc9 patchclamp amplifiers and the pulse patchclamp program. Browse our extensive list of products to the right. The digital control of all amplifier functions has been designed with two advantages in mind. Although either two epc 10 double or three epc 10 triple amplifiers are combined in a single hous. Overload of amplifiers obvious in practical use due to the loss of proper transient cancellation will occur if larger pulses are applied, unless the %comp setting is reduced. Propofol regulates the surface expression of gabaa. The epc 9 patch clamp amplifier is a digitally controlled analog device for recording currents in membrane patches and in small cells. Id love to hear peoples ideasexperiences for good ways to acquire used. The epc 800 usb can be controlled with any data acquisition interface and can be supported, not only by heka software, but also with thirdparty data acquisition programs. The pipettes were firepolished directly before the experiments and had a resistance of 3 to 7 m.
G23 instruments have developed a bnc fitting h which is rigid with the holder once connected to the headstage. We present experimental evidence of such errors, and demonstrate that they derive from pca current. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system. With conventional patch clamp amplifiers the user rarely makes parameter changes during a sensitive recording. Heka elektronik provides hardware and software for data acquisition and analysis. In this paper we show that the measurements obtained in this way are affected by errors due to the electronic design of the pca input section.
All amplifiers epc 10 usb and epc 800 usb come with our standard pipette holder for glass capillaries with an outer diameter of 1. Epc 10 usb single patch clamp amplifier the epc 10 usb is a complete data acquisition system, which can be used with hekas patchmaster software. In the following, we describe the setup for two different patch clamp amplifiers, the heka epc 7 heka electronics, darmstadt, germany and the axopatch 200b axon instrumentsmolecular devices, sunnyvale, ca, u. The patchclamp amplifiers were epc 7 list, darmstadt, germany in all voltage and currentclamp experiments. A detailed stepbystep description of the standard patch clamp protocol and labome survey results for vibratomes and patchclamp amplifiers. Epc 7 patchclamp amplifiers hekaelectronik, darmstadt, germany were used for recording without series resistance compensation 20 mv. Heka elektronik patch clamp amplifiers, potentiostats.
Winwcp is a data acquisition and analysis program for handling signals from wholecell electrophysiological experiments. Epc 10 usb single patch clamp amplifier the epc 10 usb is a complete. Journal of neuroscwnce methodv, 31 1990 8991 89 elsevier nsm 01028 a pipette holder for use in patchclamp measurements henk p. With conventional patchclamp amplifiers the user rarely makes parameter changes during a sensitive recording.
Most npi electronic amplifiers and dagan amplifiers have headstages with a bnc fitting. Similarly in the epc 9, digital artifacts are expected only when parameters are changed. Epc 10 usb single patch clamp amplifier bronjo medi. A pipette holder for use in patchclamp measurements. Ill need everything from a pipetter puller to a faraday cage and an air table. It helps in currentclamp recordings, field potential recordings in ion channel and highspeed current clamp recording within the same head stage.
Continuing the tradition of providing the worlds best electrophysiology amplifiers, the epc 10 usb double, triple, and quadro patch clamp amplifiers are the optimal instruments for performing double or triple patch experiments. At present, most laborato ries utilize either a list epc 7 or an axon axopatch series 1. Epc 10, heka,patch clamp,singapore,amplifiers bronjo medi. Firstly, if a computer controls the functions of the amplifier, then the data acquisition program can access and store variables that describe all of the settings during an experiment.